The comparative study on the effect of the locally made black soap and Dudu osun soap on dermatophytes isolated from barbing equipment using agar well diffusion method. The fungi isolated from the barbing equipment are Trichophyton rubrum (56.25%), Microsporum canis(28.13%), and Trichophyton mentagrophytes (15.63%). The isolates were treated with Dudu Osun and Black soap, the result showed that Trichophyton rubrum was resistance to 100% concentration of dudu soap but has a high growth level for 200, 300, and 400 concentration. In its treatment with black soap it was sensitive to all concentration. Trichophyton mentagrophyteswas sensitive to 100% and 400% concentration but has a high growth at 200% and 300% concentration in the treatment of dudu soap. It showed sensitivity at 200 and 300 concentation and a high growth at 100 and 400 concentration for the treatment of black soap treatment.Microsporum canis was sensitive to dudu soap treatment at 100% and had a high growth at 200%, 300%, and 400% concentration. In its treatment with black soap. It showed sensitive results at 200% and 300% but a highly growth on 100% and 400%. All controls had maximum growth. The results obtained revealed that the traditional black soap exhibited a high level of antimicrobial activities resulting in greater zones of inhibition against dudu osun soap. Hence, African black soap through this study can thus, be recommended for use since it has potentials of treating hair and skin diseases.