Mushrooms are edible fungi belonging to class Basidiomycetes. Most species of mushroom are popular and widely cultivated throughout the world owing to their simple and low cost production technology and higher biological efficiency. This study on cultivation of mushroom using different substrates adopted a culture method in which the mushroom was cultured on sawdust, corn cob and the mixture of both substrates. The parameter measured were subjected to one way analysis of variance and the experimental result was found to be significant at P<0.05% level of significance. Result gotten showed that all the substrates used supports the growth of Ganodermalucidum(mushroom), but sawdust was found to perform better in supporting the growth of the fungi. Hence, it is concluded from the study that mushroom grows better in sawdust and it’s a good substrate for the cultivation of mushroom especiallyGanodermalucidum. It is recommended in this study that sawdust substrate should be used for the cultivation of mushroom in order to obtain adequate growth especially forGanodermalucidum.