Medicinal plants have biologically compounds which are used for treating various human diseases and also play an important role in curing. Phytochemicals have two categories i.e., primary and secondary constituents. Primary constituents involve chlorophyll, proteins sugar and amino acids whereas secondary constituents contain terpenoids and alkaloids. Due to the presence of these secondary constituents medicinal plants show antifungal, antibacterial and anti-inflammation activities. The present study was designed to investigate Phytochemicals screening and antifungal studies of euphoria hirta leaves and root extract .screening revealed the presence of alkaloid, flavonoid, saponin, terpenoid, steroid and sterols in the extracts of aerial part of Euphorbia hirta. Methanol, chloroform and hexane extract of leaf and fruit were tested against Proteus mirabilis, Listeria monocytogenes, Clostridium absonum, Aspergillus niger, Aspergillus fumigates, Arthrographis cuboidea by the agar disc diffusion method.