The study examined the extent of availability and utilization of web-based tools in Nigerian Universities. A descriptive cross sectional survey research design was used for the study. Nine research questions were posed and six hypotheses formulated to guide the study. The population of the study consists of 35,680 lecturers and students in federal, state, and private universities in the study area. The sample is 395 lecturers and students, a proportionate stratified sampling technique was used to group the respondents into departments after which simple random sampling was used to draw the sample from the strata. The instruments for data collection were a checklist and questionnaires which were validated and internal consistency reliability coefficients determined using cronbach alpha technique; Data were analyzed using the IBM Statistical Package for Social Sciences version 2.0. Percentage, mean and standard deviation were used to answer the research questions, while ANOVA was used to test the hypotheses at 0.05 level of significance. It was found that Web-based tools are more available for effective delivery of instruction in State and Private Universities than Federal Universities in Nigerian; resources such as internet services, web-based learning software, personal laptops, scanners, smart boards, online library are available to a high extent.