This project work is design to investigate the effect of teachers’ qualification and instructional materials on Basic science students’ academic performance in Ukwuani LGA of Delta State, Nigeria. On the basis of this, four research hypotheses were formulated and tested. A total of one hundred (100) copies of questionnaire were distributed to one hundred (100) sampled students from five (5) different schools to elicit their responses. The instrument used in the study was questionnaire, the Chi-Square statistics was used to analyze the data collected from respondents. The findings include the following; it is evident there is a significant relationship between teachers’ qualification/experience and students’ performance in basic science, it is evident there is a significant relationship between laboratory facilities and the effective teaching and learning of basic science, it is evident there is a significant relationship between teachers’ method of teaching basic science and students’ performance, it is evident there is a significant relationship between teachers’ gender in teaching basic science and students’ performance. Moreover, in all findings; the table shows that there are relationships in all. Based on these findings, recommendations were made.